| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1211 | Mesorhizobium japonicum | TGCCTGCGACATCAGCAA | ACACCGAGCCGTTGTTGAT | 59.97 | 59.93 | 260 | 63 |
100.00%
|
100.00%
|
| Primer 1212 | Mesorhizobium japonicum | TCGTCGTTGCGCTGAACT | TTGAGAGCATCGAGACCGG | 59.97 | 59.19 | 274 | 63 |
100.00%
|
100.00%
|
| Primer 1213 | Mesorhizobium japonicum | GCCATTGCTTCGCGACAT | GCGCATCGGCACAATCAA | 59.21 | 59.52 | 233 | 63 |
100.00%
|
100.00%
|
| Primer 1214 | Mesorhizobium japonicum | TCAGCATCGGATTGCCTGG | TTTCGCCAGTGCAGCAGT | 60.15 | 60.20 | 209 | 63 |
100.00%
|
100.00%
|
| Primer 1215 | Mesorhizobium japonicum | ACGCGAAGACTGGTTCCAG | AGGCACAGCTTTCGCAGT | 60.01 | 59.89 | 245 | 63 |
100.00%
|
100.00%
|
| Primer 1216 | Mesorhizobium japonicum | TCACGCGAAGACTGGTTCC | AGGCACAGCTTTCGCAGT | 60.01 | 59.89 | 247 | 63 |
100.00%
|
100.00%
|
| Primer 1217 | Mesorhizobium japonicum | ATCGGTCTGGTCAATCGCC | ACACATCCTGGCATTCCGG | 59.86 | 60.08 | 160 | 63 |
100.00%
|
100.00%
|
| Primer 1218 | Mesorhizobium japonicum | AAGCCGAATTCCCCTGGTG | ACAACAGCACCAGGATCGG | 60.00 | 60.00 | 273 | 63 |
100.00%
|
100.00%
|
| Primer 1219 | Mesorhizobium japonicum | AGCTGTTCGAGGTTTCCGG | ATGCTGATGGCGATGGCA | 60.00 | 59.81 | 253 | 63 |
100.00%
|
100.00%
|
| Primer 1220 | Mesorhizobium japonicum | ACTGATCGAGGCACGCAA | TGAAAGCGGACATCGGCA | 59.34 | 59.65 | 285 | 63 |
100.00%
|
100.00%
|